1. Exogenous DNA does not passively enter E. coli cells that are not competent. What treatment do cells require to be competent? During our processing, we cool and then heat up the cells for a certain amount of time in order for the DNA to be able to enter the cell.
2. Why doesn’t the recovery broth used in this experiment contain ampicillin? The recovery borther used in this experiment does not contain ampicillin because it would kill the bacteria cell, therefore we would not be able to get any results.
3. What evidence do you have that transformation was successful? Evidence I have that transformation was successful (for Marcus's and Haison's) correct lab was that the plates glowed when it was under the UV light and you can see the colonies. There was also no colonies on the control plate that contained AMP.
4. What are some reasons why transformation may not be successful? Reasons for unsuccessful transformation is not streaking the agar on the plate correctly, just like what my partner and I did. Another reason is if the process of the heat shock therapy didn't process correctly, the DNA wouldn't be able to enter the cells. Lastly, if you mix the -DNA AND +DNA, you will get odd results, for example, no results at all
5. What is the source of the fluorescence? Why are some cells fluorescent and other cells not fluorescent? The course of the fluorescence is the pGFP. It is the reason the cells glow, and also is AMP resistant depending of the following of the procedures are done correctly.
2. Why doesn’t the recovery broth used in this experiment contain ampicillin? The recovery borther used in this experiment does not contain ampicillin because it would kill the bacteria cell, therefore we would not be able to get any results.
3. What evidence do you have that transformation was successful? Evidence I have that transformation was successful (for Marcus's and Haison's) correct lab was that the plates glowed when it was under the UV light and you can see the colonies. There was also no colonies on the control plate that contained AMP.
4. What are some reasons why transformation may not be successful? Reasons for unsuccessful transformation is not streaking the agar on the plate correctly, just like what my partner and I did. Another reason is if the process of the heat shock therapy didn't process correctly, the DNA wouldn't be able to enter the cells. Lastly, if you mix the -DNA AND +DNA, you will get odd results, for example, no results at all
5. What is the source of the fluorescence? Why are some cells fluorescent and other cells not fluorescent? The course of the fluorescence is the pGFP. It is the reason the cells glow, and also is AMP resistant depending of the following of the procedures are done correctly.